Discover our extensive collection of clinical studies on Chaga mushrooms (Inonotus obliquus). Learn how Chaga supports immune health, fights tumors, reduces inflammation, and aids in diabetes management. Explore the latest research and evidence-based benefits of Chaga, backed by thorough scientific investigations, to enhance your overall well-being.

• Alhallaf W, Perkins LB. The Anti-Inflammatory Properties of Chaga Extracts Obtained by Different Extraction Methods against LPS-Induced RAW 264.7. Molecules. 2022 Jun 30;27(13):4207. doi: 10.3390/molecules27134207. PMID: 35807453; PMCID: PMC9268247.

• Zheng W, Miao K, Liu Y, Zhao Y, Zhang M. Phenolics from Inonotus obliquus and their antioxidant and antitumor activities. Food Funct. 2018;9(7):3420-3431. doi:10.1039/c8fo00090a

• Wang Y, Liu Y, Du X, Ma H, Yao J. Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice. AMB Express. 2020;10(1):130. doi:10.1186/s13568-020-01068-4

• Choi JY, Shin KM, Kim HM, et al. Antioxidative effects of Phellinus linteus and red ginseng extracts on oxidative stress-induced DNA damage. J Med Food. 2006;9(1):35-42. doi:10.1089/jmf.2006.9.35

• Shen D, Feng Y, Zhang X, Liu J, Gong L, Liao H, Li R. In Vitro Immunomodulatory Effects of Inonotus obliquus Extracts on Resting M0 Macrophages and LPS-Induced M1 Macrophages.Evid Based Complement Alternat Med. 2022 Apr 21;2022:8251344. doi: 10.1155/2022/8251344. PMID: 35497923; PMCID: PMC9050302.

• Yun JS, Pahk JW, Lee JS, Shin WC, Lee SY, Hong EK. Inonotus obliquus protects against oxidative stress-induced apoptosis and premature senescence. Mol Cells. 2011 May;31(5):423-9. doi: 10.1007/s10059-011-0256-7. Epub 2011 Feb 22. PMID: 21359681; PMCID: PMC3887607.

• Vincenti, M.P., Brinckerhoff, C.E. Transcriptional regulation of collagenase (MMP-1, MMP-13) genes in arthritis: integration of complex signaling pathways for the recruitment of gene-specific transcription factors. Arthritis Res Ther 4, 157 (2002). https://doi.org/10.1186/ar401

• ScienceDaily. (2021, January 14). Even skin shielded from the sun accumulates genomic DNA changes from UV light. ScienceDaily. Retrieved November 3, 2022, from https://www.sciencedaily.com/releases/2021/01/210114163929.htm

• Eid JI, Al-Tuwaijri MM, Mohanty S, Das B. Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes. Heliyon. 2021 Feb 4;7(2):e06003. doi: 10.1016/j.heliyon.2021.e06003. Erratum in: Heliyon. 2021 Jun 08;7(6):e07235. PMID: 33598573; PMCID: PMC7868817.

• Majtan J, Jesenak M. β-Glucans: Multi-Functional Modulator of Wound Healing. Molecules. 2018 Apr 1;23(4):806. doi: 10.3390/molecules23040806. PMID: 29614757; PMCID: PMC6017669.

• Arata S, Watanabe J, Maeda M, Yamamoto M, Matsuhashi H, Mochizuki M, Kagami N, Honda K, Inagaki M. Continuous intake of the Chaga mushroom (Inonotus obliquus) aqueous extract suppresses cancer progression and maintains body temperature in mice. Heliyon. 2016 May 12;2(5):e00111. doi: 10.1016/j.heliyon.2016.e00111. PMID: 27441282; PMCID: PMC4946216.

• Lee MG, Kwon YS, Nam KS, Kim SY, Hwang IH, Kim S, Jang H. Chaga mushroom extract induces autophagy via the AMPK-mTOR signaling pathway in breast cancer cells. J Ethnopharmacol. 2021 Jun 28;274:114081. doi: 10.1016/j.jep.2021.114081. Epub 2021 Mar 30. PMID: 33798660.

• Géry A, Dubreule C, André V, Rioult JP, Bouchart V, Heutte N, Eldin de Pécoulas P, Krivomaz T, Garon D. Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B). Integr Cancer Ther. 2018 Sep;17(3):832-843. doi: 10.1177/1534735418757912. Epub 2018 Feb 27. PMID: 29484963; PMCID: PMC6142110.

• Fulda S. Betulinic Acid for cancer treatment and prevention. Int J Mol Sci. 2008 Jun;9(6):1096-1107. doi: 10.3390/ijms9061096. Epub 2008 Jun 27. PMID: 19325847; PMCID: PMC2658785. 

• Choi SY, Hur SJ, An CS, Jeon YH, Jeoung YJ, Bak JP, Lim BO. Anti-inflammatory effects of Inonotus obliquus in colitis induced by dextran sodium sulfate. J Biomed Biotechnol. 2010;2010:943516. doi: 10.1155/2010/943516. Epub 2010 Mar 10. PMID: 20300439; PMCID: PMC2840610.

• Alhallaf W, Perkins LB. The Anti-Inflammatory Properties of Chaga Extracts Obtained by Different Extraction Methods against LPS-Induced RAW 264.7. Molecules. 2022 Jun 30;27(13):4207. doi: 10.3390/molecules27134207. PMID: 35807453; PMCID: PMC9268247.

• Wang J, Wang C, Li S, Li W, Yuan G, Pan Y, Chen H. Anti-diabetic effects of Inonotus obliquus polysaccharides in streptozotocin-induced type 2 diabetic mice and potential mechanism via PI3K-Akt signal pathway. Biomed Pharmacother. 2017 Nov;95:1669-1677. doi: 10.1016/j.biopha.2017.09.104. Epub 2017 Oct 6. PMID: 28954386.

• Pan HH, Yu XT, Li T, Wu HL, Jiao CW, Cai MH, Li XM, Xie YZ, Wang Y, Peng T. Aqueous extract from a Chaga medicinal mushroom, Inonotus obliquus (higher Basidiomycetes), prevents herpes simplex virus entry through inhibition of viral-induced membrane fusion. Int J Med Mushrooms. 2013;15(1):29-38. doi: 10.1615/intjmedmushr.v15.i1.40. PMID: 23510282.

• Polkovnikova MV, Nosik NN, Garaev TM, Kondrashina NG, Finogenova MP, Shibnev VA. [A study of the antiherpetic activity of the chaga mushroom (Inonotus obliquus) extracts in the Vero cells infected with the herpes simplex virus]. Vopr Virusol. 2014 Mar-Apr;59(2):45-8. Russian. PMID: 25069286.

• Yoon TJ, Lee SJ, Kim EY, Cho EH, Kang TB, Yu KW, Suh HJ. Inhibitory effect of chaga mushroom extract on compound 48/80-induced anaphylactic shock and IgE production in mice. Int Immunopharmacol. 2013 Apr;15(4):666-70. doi: 10.1016/j.intimp.2013.03.015. Epub 2013 Mar 25. PMID: 23535020.

• Szychowski KA, Skóra B, Pomianek T, Gmiński J. Inonotus obliquus - from folk medicine to clinical use. J Tradit Complement Med. 2020 Aug 22;11(4):293-302. doi: 10.1016/j.jtcme.2020.08.003. PMID: 34195023; PMCID: PMC8240111.

• Nguyet TMN, Lomunova M, Le BV, Lee JS, Park SK, Kang JS, Kim YH, Hwang I. The mast cell stabilizing activity of Chaga mushroom critical for its therapeutic effect on food allergy is derived from inotodiol. Int Immunopharmacol. 2018 Jan;54:286-295. doi: 10.1016/j.intimp.2017.11.025. Epub 2017 Nov 24. PMID: 29175507.

• https://www.webmd.com/diet/health-benefits-chaga-mushrooms

• Alhallaf W, Perkins LB. The Anti-Inflammatory Properties of Chaga Extracts Obtained by Different Extraction Methods against LPS-Induced RAW 264.7. Molecules. 2022 Jun 30;27(13):4207. doi: 10.3390/molecules27134207. PMID: 35807453; PMCID: PMC9268247.

• Zheng W, Miao K, Liu Y, Zhao Y, Zhang M. Phenolics from Inonotus obliquus and their antioxidant and antitumor activities. Food Funct. 2018;9(7):3420-3431. doi:10.1039/c8fo00090a

• Wang Y, Liu Y, Du X, Ma H, Yao J. Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice. AMB Express. 2020;10(1):130. doi:10.1186/s13568-020-01068-4

• Choi JY, Shin KM, Kim HM, et al. Antioxidative effects of Phellinus linteus and red ginseng extracts on oxidative stress-induced DNA damage. J Med Food. 2006;9(1):35-42. doi:10.1089/jmf.2006.9.35

• Shen D, Feng Y, Zhang X, Liu J, Gong L, Liao H, Li R. In Vitro Immunomodulatory Effects of Inonotus obliquus Extracts on Resting M0 Macrophages and LPS-Induced M1 Macrophages.Evid Based Complement Alternat Med. 2022 Apr 21;2022:8251344. doi: 10.1155/2022/8251344. PMID: 35497923; PMCID: PMC9050302.

• Yun JS, Pahk JW, Lee JS, Shin WC, Lee SY, Hong EK. Inonotus obliquus protects against oxidative stress-induced apoptosis and premature senescence. Mol Cells. 2011 May;31(5):423-9. doi: 10.1007/s10059-011-0256-7. Epub 2011 Feb 22. PMID: 21359681; PMCID: PMC3887607.

• Vincenti, M.P., Brinckerhoff, C.E. Transcriptional regulation of collagenase (MMP-1, MMP-13) genes in arthritis: integration of complex signaling pathways for the recruitment of gene-specific transcription factors. Arthritis Res Ther 4, 157 (2002). https://doi.org/10.1186/ar401

• ScienceDaily. (2021, January 14). Even skin shielded from the sun accumulates genomic DNA changes from UV light. ScienceDaily. Retrieved November 3, 2022, from https://www.sciencedaily.com/releases/2021/01/210114163929.htm

• Eid JI, Al-Tuwaijri MM, Mohanty S, Das B. Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes. Heliyon. 2021 Feb 4;7(2):e06003. doi: 10.1016/j.heliyon.2021.e06003. Erratum in: Heliyon. 2021 Jun 08;7(6):e07235. PMID: 33598573; PMCID: PMC7868817.

• Majtan J, Jesenak M. β-Glucans: Multi-Functional Modulator of Wound Healing. Molecules. 2018 Apr 1;23(4):806. doi: 10.3390/molecules23040806. PMID: 29614757; PMCID: PMC6017669.

• Arata S, Watanabe J, Maeda M, Yamamoto M, Matsuhashi H, Mochizuki M, Kagami N, Honda K, Inagaki M. Continuous intake of the Chaga mushroom (Inonotus obliquus) aqueous extract suppresses cancer progression and maintains body temperature in mice. Heliyon. 2016 May 12;2(5):e00111. doi: 10.1016/j.heliyon.2016.e00111. PMID: 27441282; PMCID: PMC4946216.

• Lee MG, Kwon YS, Nam KS, Kim SY, Hwang IH, Kim S, Jang H. Chaga mushroom extract induces autophagy via the AMPK-mTOR signaling pathway in breast cancer cells. J Ethnopharmacol. 2021 Jun 28;274:114081. doi: 10.1016/j.jep.2021.114081. Epub 2021 Mar 30. PMID: 33798660.

• Géry A, Dubreule C, André V, Rioult JP, Bouchart V, Heutte N, Eldin de Pécoulas P, Krivomaz T, Garon D. Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B). Integr Cancer Ther. 2018 Sep;17(3):832-843. doi: 10.1177/1534735418757912. Epub 2018 Feb 27. PMID: 29484963; PMCID: PMC6142110.

• Fulda S. Betulinic Acid for cancer treatment and prevention. Int J Mol Sci. 2008 Jun;9(6):1096-1107. doi: 10.3390/ijms9061096. Epub 2008 Jun 27. PMID: 19325847; PMCID: PMC2658785.

• Choi SY, Hur SJ, An CS, Jeon YH, Jeoung YJ, Bak JP, Lim BO. Anti-inflammatory effects of Inonotus obliquus in colitis induced by dextran sodium sulfate. J Biomed Biotechnol. 2010;2010:943516. doi: 10.1155/2010/943516. Epub 2010 Mar 10. PMID: 20300439; PMCID: PMC2840610.

• Alhallaf W, Perkins LB. The Anti-Inflammatory Properties of Chaga Extracts Obtained by Different Extraction Methods against LPS-Induced RAW 264.7. Molecules. 2022 Jun 30;27(13):4207. doi: 10.3390/molecules27134207. PMID: 35807453; PMCID: PMC9268247.

• Wang J, Wang C, Li S, Li W, Yuan G, Pan Y, Chen H. Anti-diabetic effects of Inonotus obliquus polysaccharides in streptozotocin-induced type 2 diabetic mice and potential mechanism via PI3K-Akt signal pathway. Biomed Pharmacother. 2017 Nov;95:1669-1677. doi: 10.1016/j.biopha.2017.09.104. Epub 2017 Oct 6. PMID: 28954386.

• Pan HH, Yu XT, Li T, Wu HL, Jiao CW, Cai MH, Li XM, Xie YZ, Wang Y, Peng T. Aqueous extract from a Chaga medicinal mushroom, Inonotus obliquus (higher Basidiomycetes), prevents herpes simplex virus entry through inhibition of viral-induced membrane fusion. Int J Med Mushrooms. 2013;15(1):29-38. doi: 10.1615/intjmedmushr.v15.i1.40. PMID: 23510282.

• Polkovnikova MV, Nosik NN, Garaev TM, Kondrashina NG, Finogenova MP, Shibnev VA. [A study of the antiherpetic activity of the chaga mushroom (Inonotus obliquus) extracts in the Vero cells infected with the herpes simplex virus]. Vopr Virusol. 2014 Mar-Apr;59(2):45-8. Russian. PMID: 25069286.

• Yoon TJ, Lee SJ, Kim EY, Cho EH, Kang TB, Yu KW, Suh HJ. Inhibitory effect of chaga mushroom extract on compound 48/80-induced anaphylactic shock and IgE production in mice. Int Immunopharmacol. 2013 Apr;15(4):666-70. doi: 10.1016/j.intimp.2013.03.015. Epub 2013 Mar 25. PMID: 23535020.

• Szychowski KA, Skóra B, Pomianek T, Gmiński J. Inonotus obliquus - from folk medicine to clinical use. J Tradit Complement Med. 2020 Aug 22;11(4):293-302. doi: 10.1016/j.jtcme.2020.08.003. PMID: 34195023; PMCID: PMC8240111.

• Nguyet TMN, Lomunova M, Le BV, Lee JS, Park SK, Kang JS, Kim YH, Hwang I. The mast cell stabilizing activity of Chaga mushroom critical for its therapeutic effect on food allergy is derived from inotodiol. Int Immunopharmacol. 2018 Jan;54:286-295. doi: 10.1016/j.intimp.2017.11.025. Epub 2017 Nov 24. PMID: 29175507.

• https://www.webmd.com/diet/health-benefits-chaga-mushrooms